When a research peptide is described as “HPLC verified” or “HPLC + MS tested”, two complementary analytical techniques are doing the work. They are often mentioned together, but they measure different things. Knowing the distinction makes every Certificate of Analysis easier to interpret.<\/p>\n
\u9ad8\u901f\u6db2\u4f53\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306f\u3001\u6eb6\u89e3\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u9ad8\u5727\u4e0b\u3067\u5145\u586b\u30ab\u30e9\u30e0\u306b\u62bc\u3057\u8fbc\u307f\u307e\u3059\u3002\u7570\u306a\u308b\u5206\u5b50\u306f\u3001\u30ab\u30e9\u30e0\u6750\u6599\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u306e\u5f37\u3055\u306b\u5fdc\u3058\u3066\u7570\u306a\u308b\u901f\u5ea6\u3067\u30ab\u30e9\u30e0\u3092\u901a\u904e\u3059\u308b\u305f\u3081\u3001\u4e3b\u8981\u306a\u30da\u30d7\u30c1\u30c9\u304c\u95a2\u9023\u3059\u308b\u4e0d\u7d14\u7269\u304b\u3089\u5206\u96e2\u3055\u308c\u307e\u3059\u3002\u691c\u51fa\u5668\u306f\u5404\u6210\u5206\u3092\u30d4\u30fc\u30af\u3068\u3057\u3066\u8a18\u9332\u3057\u307e\u3059\u3002\u30e1\u30a4\u30f3\u30d4\u30fc\u30af\u306e\u4e0b\u306e\u9762\u7a4d\u3092\u5408\u8a08\u306b\u5bfe\u3057\u3066\u7a4d\u5206\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u5206\u6790\u8005\u306f\u7d14\u5ea6\u30d1\u30fc\u30bb\u30f3\u30c6\u30fc\u30b8\u3092\u8a08\u7b97\u3057\u307e\u3059\u3002<\/p>\n
HPLC is therefore a purity<\/strong> tool. It tells you how much of the sample is the dominant compound versus everything else \u2014 but on its own it does not prove what that dominant compound actually is.<\/p>\n Mass Spectrometry (MS) ionises the molecules and measures their mass-to-charge ratio with high precision. For a peptide, the measured mass is compared against the theoretical mass derived from its amino-acid sequence. A close match confirms identity<\/strong>: the molecule really is the sequence on the label, and not a different peptide of similar size.<\/p>\n MS is an identity tool. It is exceptionally good at confirming “this is the right molecule” but is not the primary way purity is quantified.<\/p>\n Consider the gaps each technique leaves:<\/p>\n Used together, HPLC answers “how pure?” and MS answers “is it the right molecule?”. A COA that includes both gives a far more complete picture than either in isolation, which is why “HPLC + MS” has become the practical standard for research-grade peptide documentation.<\/p>\n \u7d44\u307f\u5408\u308f\u305b\u305f\u30ec\u30dd\u30fc\u30c8\u3067\u306f\u3001HPLC \u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0 (\u7406\u60f3\u7684\u306b\u306f\u3001\u6307\u5b9a\u3055\u308c\u305f\u7d14\u5ea6\u30d1\u30fc\u30bb\u30f3\u30c6\u30fc\u30b8\u3092\u6301\u3064\u304d\u308c\u3044\u306a\u4e3b\u8981\u30d4\u30fc\u30af) \u3068\u3001\u8a08\u7b97\u3055\u308c\u305f\u8cea\u91cf\u306e\u96a3\u306b\u89b3\u6e2c\u3055\u308c\u305f\u8cea\u91cf\u3092\u793a\u3059 MS \u30b9\u30da\u30af\u30c8\u30eb\u307e\u305f\u306f\u8868\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002\u6e2c\u5b9a\u3055\u308c\u305f\u8cea\u91cf\u304c\u88fd\u54c1\u4ed5\u69d8\u306e\u5206\u5b50\u91cf\u3068\u4e00\u81f4\u3057\u3066\u3044\u308b\u3053\u3068\u3001\u304a\u3088\u3073\u7d14\u5ea6\u306e\u6570\u5024\u304c\u6ce8\u6587\u3057\u305f\u30b0\u30ec\u30fc\u30c9\u3092\u6e80\u305f\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n 2 \u3064\u306e\u624b\u6cd5\u304c\u982d\u306e\u4e2d\u3067\u660e\u78ba\u306b\u533a\u5225\u3055\u308c\u308b\u3068\u3001\u7814\u7a76\u7528\u30da\u30d7\u30c1\u30c9 COA \u306f\u6570\u5b57\u306e\u58c1\u3067\u306f\u306a\u304f\u306a\u308a\u3001\u8fc5\u901f\u304b\u3064\u78ba\u5b9f\u306a\u691c\u8a3c\u304c\u53ef\u80fd\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n \u53c2\u7167\u3055\u308c\u3066\u3044\u308b\u5316\u5408\u7269\u306f\u3001\u5b9f\u9a13\u5ba4\u3067\u306e\u4f7f\u7528\u306e\u307f\u3092\u76ee\u7684\u3068\u3057\u305f\u7814\u7a76\u30b0\u30ec\u30fc\u30c9\u306e\u6a19\u6e96\u7269\u8cea\u3067\u3042\u308a\u3001\u4eba\u9593\u3084\u52d5\u7269\u306e\u6d88\u8cbb\u3092\u76ee\u7684\u3068\u3057\u305f\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/em><\/p>","protected":false},"excerpt":{"rendered":" HPLC \u3068\u8cea\u91cf\u5206\u6790\u306f\u3001\u30da\u30d7\u30c1\u30c9\u306b\u95a2\u3059\u308b 2 \u3064\u306e\u7570\u306a\u308b\u8cea\u554f\u306b\u7b54\u3048\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u304c\u4f55\u3092\u6e2c\u5b9a\u3059\u308b\u306e\u304b\u3092\u7406\u89e3\u3059\u308b\u3068\u3001COA \u304c\u8aad\u307f\u3084\u3059\u304f\u306a\u308a\u307e\u3059\u3002<\/p>","protected":false},"author":1,"featured_media":240,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[],"class_list":["post-26","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-quality-testing"],"yoast_head":"\n\u8cea\u91cf\u5206\u6790\u304c\u6e2c\u5b9a\u3059\u308b\u3082\u306e: \u30a2\u30a4\u30c7\u30f3\u30c6\u30a3\u30c6\u30a3<\/h2>\n
Why both are used together<\/h2>\n
\n
Reading the two results side by side<\/h2>\n
Practical takeaways<\/h2>\n
\n